SHROOQ N. KHUDHER, KHAIRALLAH A. S. MOHAMMED1, TALAL H. NOON AND QAIS R. LAHHOB
Department of Agricultural Economics, Graduate School, Faculty of Agriculture, Universitas Gadjah Mada (UGM),
Bulaksumur, Caturtunggal, Depok, Sleman, 55281, Yogyakarta, Indonesia
*(e-mail: isnaini.rohmawati@mail.ugm.ac.id; Mobile: 628 78154 26682)
(Received: January 8, 2023; Accepted: February 14, 2023)
ABSTRACT
Infertility can be a sign of the condition, and celiac disease, in particular, can lead to repeated abortions. The primary goal of this study was to look into the link between celiac disease and abortion, and in particular to evaluate immunological markers and genetic allele in miscarriage female. This study included
45 patients and 34 healthy adults ranging in age from 24 to 55 years. Five milliliters of blood was obtained from each participant and tested for tTg-IgA, IL-17A and HLA-DQ2/DQ8 using ELISA and RT PCR, respectively. Females with celiac disease had higher rates of abortion than controls (35.56% vs. 8.82%; P = 0.007, OD = 5.70, CI = 1.63 to 19.6 ), in addition significant difference (P< 0.001) between patients (mean±SD) 94.1±101, 369±638 and control 4.50±1.91, 15.0±3.41 were recorded in serum tTg-IgA and IL_17A, respectively. Furthermore, a high genetic propensity to CD was shown to be connected with the HLA class-II allele. The findings indicated that 7(15.56%) of 45 patients were positive for DQ8, with no significant difference between patients and controls, but 37 (82.22%) of 45 patients were DQ2 positive, with a significant difference between patients and controls. The results confirmed a marked association among HLA-DQ2 and history of abortion in woman ciliac patients. Increased Ttg-IgA and IL-17A tiers in female patients may play a crucial position inside the severity of the circumstance.
Key words : Celiac disease, real time PCR, IL_17A, IgA-tTg, HLA-DQ2/8 alleles