RAMAN BALA , PUNEET BENIWAL*, JITENDER SINGH LAURA AND VIJAY SINGH BENIWAL
Department of Agriculture, Guru Nanak College, Budhlada, Mansa-151 502 (Punjab), India
*(e-mail : firstname.lastname@example.org; 9812113187)
(Received : May 21, 2020; Accepted : July 18, 2020)
The study was focused to develop an efficient method using cotyledonary node explants from two month old seedlings of Simmondsia chinensis. Among the different concentrations (0.5, 1.0, 1.5 and 2.0 mg/l) of BAP tested, highest per cent (98.5%) of shoot regeneration with maximum number of shoot buds (5.82 shoots/explant) was observed on MS medium supplemented with 1.0 mg/l of BAP with 40 mg/l adenine sulphate. For multiplication and elongation of shoots, highest number of shoots (8.90 shoots/explant) on MS medium contained 1.0 mg/l BAP+0.5 mg/l NAA+40 mg/l adenine sulphate with a proliferation rate of 99.2% with average shoot length of 4.97 cm. Among the various concentrations used, the highest frequency (97.3%) of root induction was noticed on 3.0 mg/l IBA with 200 mg/l activated charcoal. Well rooted plantlets were successfully hardened and acclimatized with 85 % survival rate.
Key words : Activated charcoal, adenine sulphate, cotyledonary node, regeneration, jojoba