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Histopathological Changes Resulting from the Effect of Nano-graphene Oxide on the Liver in Laboratory Rats

Department of Biology, College of Education for Pure Sciences, University of Thi-Qar, Iraq
*(e-mail :; Mobile : +964 75009 84372)
(Received : May 25, 2021; Accepted : May 31, 2022)


The current study included the use of 48 white male laboratory rats which were divided into six groups,
with eight rats in each group. These groups were treated with a concentration of 20, 30, 40, 50 and 60
mg/kg nano graphene oxide. The sixth group was the control group. The results of the statistical analysis
of the study showed a significant decrease in the average body weight. On the last day of the experiment,
the results showed a significant decrease in the average liver weight when comparing between the
control group and the treated groups, central vein, hepatic sinusoidal dilatation, hepatocyte nucleus
replication, hepatocyte rupture and nucleolysis as well as thickening of the nuclei in some hepatocytes,
infiltration of inflammatory cells, hemorrhage and dissolution and necrosis of hepatocytes. In conclusion,
the results of the over-study by transmission electron microscope also showed changes at the cellular
level, represented by the rupture of the nucleus and cytoplasm, in addition to the dissolution of the
nucleus due to exposure to nano-graphene oxide.
Key words : Histopathological changes, nano-graphene oxide, liver, laboratory rats