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Investigation of the Effect of Application of Ascorbic Acid and Biofertilizers on Basil Medicinal Plant under the Influence of Drought Stress

Mohsen Hamidi, Hamidreza Tohidi Moghadam, Mohammad Nasri, Pourang Kasraie and Hamidreza Larijani
Department of Agriculture, Varamin-Ppishva Branch, Islamic Azad University, Iran
*(e-mail :; Mobile : 989128050517)
(Received : June 10, 2021; Accepted : August 12, 2021)


The present study evaluated the effect of ascorbic acid and the coexistence of mycorrhizal fungi and azospirillum bacteria on basil (Ocimum basilicum L.) under the influence of different irrigation treatments. This experiment was performed as a factorial split in the form of a randomized complete block design with three replications during two crop years 2017 and 2018 in Ghamsar village located in Shahreri city. In this experiment, irrigation as the main factor at three levels including drought stress based on 40, 70 and 100 mm evaporation from class A surface evaporation pan and biofertilizer including azospirillum growth-promoting bacteria and mycorrhizal fungi at four levels including A : no consumption; B : along with bacterial seed stimulating the growth of azospirillum; C : consumption of mycorrhizal fungus as a seed and D : concomitant use of azospirillum growth-promoting bacteria with mycorrhizal fungi in the form of malt seeds and ascorbic acid at two levels including A : non-use of ascorbic acid and B : application of ascorbic acid as a secondary factor. The highest biological yield was obtained in 40 mm drought stress and the application of mycorrhizal biological fertilizers at a rate of 7002.21 kg/ha, which was about 70% more than the treatment of 100 mm evaporation stress and no biological fertilizer consumption. The results showed that the use of ascorbic acid in drought stress conditions of 40 mm increased 45% IAA, 30% relative leaf water content and 41% decrease in malondialdehyde compared to the treatment of ascorbic acid consumption and 100 mm stress evaporation.
Key words : : Basil plant, drought stress, biological fertilizer, ascorbic acid, biological yield