K. LATHA AND H. RAJA NAIKA*
Department of Studies and Research in Biotechnology, Tumkuru University, Tumkuru-572 102 (Karnataka), India
*(e-mail : rajanaik1100@gmail.com; Mobile : 8217253664)
(Received : December 13, 2019, Accepted : February 15, 2020)
ABSTRACT
Cucumis sativus L. var. sativus belongs to Cucurbitaceae. It is the most popular vegetable as well as fruit used in Indian traditional medicine. The aim of our study was to identify bioactive compounds present in the white foamy substance. This study evaluates cytotoxic, antioxidant and Na+/K+-ATPase activity. The metabolite profiling of white foamy substance was performed using HR-LCMS coupled with a Quadrupole ion trap Mass Spectrometer. The foamy substance was subjected to the MTT assay, DPPH assay and Na+/K+-ATPase activity. The analytical data showed that presence of Triterpenes : Cucurbitacin A (0.35 ppm), Cucurbitacin-B (-5.93 ppm), Cucurbitacin-C (0.65 ppm), Cucurbitacin-E (7.17 ppm) and Cardiac glycosides : Gitoxigenin (8.33 ppm), Strophanthidin (0.96 ppm) and Digitoxigenin (-5.95 ppm) in 10 µl of loaded sample. One hundred other compounds were identified by accurate mass of Q-TOF/MS.
Key words : Cucumis sativus L. var. sativus, DPPH Assay HR-LCMSQ-TOF/MS, MTT Assay, Na+/K+– ATPase activity