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The Application of BAP and 2,4-D on the Growth of Euchresta horsfieldii (Lesch.) Benn. through in vitro Culture

ANDRIYANA SETYAWATI*, DEFRITA FITRI RAMADHANI, AMALIA TETRANI SAKYA, NANDARIYAH, ENDANG YUNIASTUTI, OKKY TALITHA AND ALFIDA MUTHIā€™AH
Department of Agrotechnology, Faculty of Agriculture, Universitas Sebelas Maret, Surakarta, 57126, Central Java, Indonesia
*(e-mail: andriyanasetyawati@staff.uns.ac.id; Mobile: +628 52939 42727)
(Received: July 20, 2023; Accepted: September 10, 2023)

ABSTRACT

Euchresta horsfieldii (Lesch.) is one of the medicinal plants having many benefits and in high demand. This study was aimed at obtaining the proper concentrations of BAP and 2,4-D for increasing the production of psychic in vitro. The study used a completely randomized design (CRD) which was arranged in a factorial design consisting of two treatment factors with 25 treatment combinations repeated three times. The addition of 2 ppm BAP and 2,4-D 0 ppm showed the best response on shoot height, number of shoots and number of leaves. Most of the emerging calluses were green with a compact callus texture. Callus appeared in all treatments, while shoots appeared mostly on media with a concentration of 2,4- D 0 ppm. Further research can be done by using embryos as explants, using different growth regulators.
Key words : Auxins, cytokinins, plant tissue, secondary metabolites