SISWANTO*, UMI KULSUM, RETNA APSARI AND AMINATUN
Department of Physics, Faculty of Science and Technology, Universitas Airlangga, Surabaya, 60115, East
*(e-mail : email@example.com; Mobile : 081230913787)
Research on the synthesis of collagen-hydroxyapatite (HA) composite scaffold as a bone replacement biomaterial using the freeze-drying method was carried out. Synthesis of this composite scaffold was carried out with freezing time variations of 2, 4 and 6 h at -80°C. Scaffold freeze-drying results were then characterized to determine the pore size of the scaffold by conducting SEM tests and osteoinduction properties through osteoblast cell differentiation test. Freezing time variation resulted in pore size variation in collagen-HA composite scaffold with pore size more than 100 µm. Increased pore size had an influence on the speed of the osteoblast cell differentiation process.
Key words : Scaffold, composites, hydroxyapatite-collagen, pores, osteoblasts