PAMELA SINGH, VIPIN KHASA*, VIPUL CHAUDHARY AND SONIA TYAGI
Department of Biotechnology, Deenbandhu Chhotu Ram University, Murthal, Sonipat-131 006 (Haryana), India
*(e-mail : vkhasasnp@gmail.com; Mobile : 98131 21824)
(Received : February 16, 2022; Accepted : April 9, 2022)
ABSTRACT
Bovine mastitis is one of the major problems faced by livestock owner and its treatment and control still
remain a challenge. The study was planned to isolate and identify bacteria responsible for the most of
the infections. Out of a total of 78 milk samples processed, 22 (28.2%) were found infected, out of which
Staphylococcus spp. remained most predominant (59.1%) followed by E. coli (36.4%) and Pseudomonas
spp. (4.5%). Antibiotic sensitivity assay revealed that Staphylococcus spp. isolates were 100% sensitive
to oxytetracycline, followed by equal sensitivity (92.3%) to chloramphenicol, bacitracin, enrofloxacin
and gentamycin. Maximum resistance was found against colistin followed by cephaperazone and
ceftizoxime. Gram negative bacteria E. coli and Pseudomonas isolates were also found carrying resistance
against bacitracin (88.9%) followed by amoxyclav (77.8%) and erythromycin (77.8%) besides ceftozoxime
(66.7%).
Key words : Mastitis, bovine, antibiotic, Staphylococcus, E. coli