ALI MOHAMMED ABED* AND RAND MUHAMMED ABDUL-HUSSEIN AL-HUSSEINI
Faculty of Science, University of Kufa, Iraq
*(e-mail : alabedy92@yahoo.com; Mobile : 009647803439614; **rand.alhusseini@uokufa.edu.iq; Mobile :
009647725728195)
(Received : June 17, 2019; Accepted : August 13, 2019)
ABSTRACT
Breast cancer is the world’s second leading cause of cancer death and is the most common cancer type in women. Most cancer-related deaths are due to metastatic tumor development. Identification of gene mediators for metastatic breast-cancer offers new potential targets. This study included paraffin blocks of 75 Iraqi women with confirmed breast cancer and 25 samples with normal breast tissues were considered as a control group. RFLP-PCR technique was performed for detection and genotyping SIPA1 gene two polymorphisms; -545 C>T (rs3741378) and -2760 G>A (rs746429). The genotype distribution of the -545C>T (rs3741378) single-bp polymorphisms of SIPA1 gene showed significant difference (P<0.05) between controls (CC : n= 23, 92%; CT : n=2, 8 %; TT : n=0) and breast cancer patients (CC : n= 19, 25.33%; CT : n=30, 40%; TT : n=26, 34.66%).The association of SIPA1 -545C>T polymorphism and pathological features of the breast cancer cases was analyzed. There were significant associations between SIPA1 -545C>T polymorphism and histologic grade, TNM staging, Lymph node metastases, and hormone receptor status
of breast cancer cases (P<0.05). The genotype distribution of the -2760 G>A (rs746429) single-bp polymorphisms of SIPA1 gene showed significant difference (P<0.05) between controls (GG : n= 20, 80%; GA : n=5, 20%; TT : n=0) and breast cancer patients (GG : n=37, 49.33%; GA : n=26, 34.66%; AA : n=12, 16%). There were significant associations between SIPA1 -2760 G>A polymorphism and histologic grade, TNM staging and lymph node metastases of breast cancer cases (P<0.05).
Key words :Breast cancer, SIPA1 gene, polymorphisms